Molecular basis of copper transport: cellular and physiological functions of Menkes and Wilson disease proteins (ATP7A and ATPB)

Eukaryotic cells prevent copper-induced, free radical damage to cell components by employing copper-binding proteins and transporters that minimize the likelihood of free copper ions existing in the cell. In the cell, copper is actively transported from the cytoplasm during the biosynthesis of secreted coppercontaining proteins and, as a protective measure, when there is an excess of copper. In humans, this is accomplished by two related copper-transporting ATPases (ATP7A and ATP7B), which are the affected genes in two distinct human genetic disorders of copper transport, Menkes disease (copper deficiency) and Wilson disease (copper toxicosis). The study of these ATPases has revealed their molecular mechanisms of copper transport and their roles in physiological copper homeostasis. Both ATP7A and ATP7B are expressed in specific brain regions and neurological abnormalities are important clinical features in Menkes and Wilson disease.

Molecular and cellular aspects of copper transport in developing mammals

Copper is an essential trace element that requires tightly regulated homeostatic mechanisms to ensure adequate supplies without any toxic effects because of the ability of the metal ion to catalyze the formation of free radicals. The Cu-ATPases, ATP7A and ATP7B, play an important role in the physiological regulation of copper. Adequate supplies of copper are particularly important in developing animals, and in humans this is illustrated by mutations of ATP7A that cause the copper deficiency condition Menkes disease, which is fatal in early childhood. In contrast, mutations in ATP7B result in the genetic toxicosis, Wilson disease. We propose that the physiological regulation of copper is accomplished mainly by the intracellular copper-regulated trafficking of the Cu-ATPases. This process allows the overall copper status in the body to be maintained when levels of copper in the diet alter. A study of the defects in mouse models of Menkes and Wilson diseases has demonstrated that both ATPases play an important role in supplying copper to the developing fetus and neonate

Effects of the density on compressive properties in cellular aluminum produced by the sintering method

The cellular aluminum materials with relative densities of 0.1"-'0.25 were fabricated by the sintering method and effects of the density on mechanical properties of the cellular aluminum were investigated by compressive tests. The cellular aluminum exhibited a plateau region with a nearly constant flow stress. The stress in the plateau region increased with increasing relative density, on the other hand, the densification strain decreased with increasing relative density. Observation of the deformed cells revealed that the cell walls were bent. Besides, the stress in the plateau region was proportional to 1.9 power of the density. These suggest that plastic collapse is dominated by bending of the cell walls for the cellular aluminum produced by the sintering method.

Speed control and policing in a cellular mobile network: speedNet

In this paper, we describe SpeedNet, a GSM network variant which resembles an ad hoc wireless mobile network where base stations keep track of the velocities of mobile users (cars). SpeedNet is intended to track mobile users and their speed passively for both speed policing and control of traffic. The speed of the vehicle is controlled in a speed critical zone by means of an electro-mechanical control system, suitably referred to as VVLS (Vehicular Velocity Limiting System). VVLS is mounted on the vehicle and responds to the command signals generated by the base station. It also determines the next base station to handoff, in order to improve the connection reliability and bandwidth efficiency of the underlying network. Robust Extended Kalman Filter (REKF) is used as a passive velocity estimator of the mobile user with the widely used proportional and integral controller speed control. We demonstrate through simulation and analysis that our prediction algorithm can successfully estimate the mobile user’s velocity with low system complexity as it requires two closest mobile base station measurements and also it is robust against system uncertainties due to the inherent deterministic nature in the mobility model.

Mobility modelling and trajectory prediction for cellular networks with mobile base stations

This paper provides mobility estimation and prediction for a variant of GSM network which resembles an adhoc wireless mobile network where base stations and users are both mobile. We propose using Robust Extended Kalman Filter (REKF)as a location heading altitude estimator of mobile user for next node (mobile-base station)in order to improve the connection reliability and bandwidth efficiency of the underlying system. Through analysis we demonstrate that our algorithm can successfully track the mobile users with less system complexity as it requires either one or two closest mobile-basestation measurements. Further, the technique is robust against system uncertainties due to inherent deterministic nature in the mobility model. Through simulation, we show the accuracy and simplicity in implementation of our prediction algorithm.

Speed control and policing in a cellular mobile network: SpeedNet

In this paper, we describe SpeedNet, a GSM network variant which resembles an ad hoc wireless mobile network where base stations (possibly other vehicles in the network) keep track of the velocities of mobile users (cars). SpeedNet is intended to track mobile users and their speed passively for both speed policing and control of traffic. The speed of the vehicle is controlled in a speed critical zone by means of an electro-mechanical control system, suitably referred to as VVLS (vehicular velocity limiting system). VVLS is mounted in the vehicle and responds to the command signals generated by the base station. It also determines the next basestation to handoff, in order to improve the connection reliability and bandwidth efficiency of the underlying network. Robust extended Kalman filter (REKF) is used as a passive velocity estimator of the mobile user with the widely used proportional and integral controller speed control. We demonstrate through simulation and analysis that our prediction algorithm can successfully estimate the mobile users velocity with low system complexity as it requires two closet mobile-base station measurement and also it is robust against system uncertainties due to the inherent deterministic nature in the mobility model.

Location based power control for mobile devices in a cellular network

This paper provides location estimation based power control strategy for cellular radio systems via a location based interference management scheme. Our approach considers the carrier-to-interference as dependent on the transmitter and receiver separation distance and therefore an accurate estimation of the precise locations can provide the power critical mobile user to control the transition power accordingly. In this fully
distributed algorithms, we propose using a Robust Extended Kalman Filter (REKF) to derive an estimate of the mobile user’s closest mobile base station from the user’s location, heading and altitude. Our analysis demonstrates that this algorithm can successfully track the mobile users with less system complexity, as it requires measurements from only one or two closest mobile base stations and hence enable the user to transmit at the rate that is sufficient for the interference management. Our power control
algorithms based on this estimation converges to the desired power trajectory. Further, the technique is robust against system uncertainties caused by the inherent deterministic nature of the mobility model. Through simulation, we show the accuracy of our prediction algorithm and the simplicity of its implementation.

Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies

A simple and sensitive HPLC method was developed to simultaneously determine CPT-11 and its major metabolite SN-38 in culture media and cell lysates. Camptothecin (CPT) was used as internal standard (I.S.). Compounds were eluted with acetonitrile–50 mM disodium hydrogen phosphate buffer containing 10 mM sodium 1-heptane-sulfonate, with the pH adjusted to 3.0 using 85% (w/v) orthophosphoric acid (27/73, v/v) by a Hyperclon ODS (C18) column (200 mm × 4.6 mm i.d.), with detection at excitation and emission wavelengths of 380 and 540 nm, respectively. The average extraction efficiencies were 96.9–108.3% for CPT-11 in culture media and 94.3–107.2% for CPT-11 in cell lysates; and 87.7–106.8% for SN-38 in culture media and 90.1–105.6% for SN-38 in cell lysates. Within- and between-day precision and accuracy varied from 0.1 to 10.3%. The limit of quantitation (precision and accuracy <20%) was 5.0 and 2.0 ng/ml for CPT-11 and 1.0 and 0.5 ng/ml for SN-38 in culture media and cell lysates, respectively. This method was successfully applied to quantitate the cellular accumulation and metabolism of CPT-11 and SN-38 in H4-II-E, a rat hepatoma cell line.

Simulation of dynamic recrystallization using random grid cellular automata

Computer simulation is a powerful tool to predict microstructure and its evolution in dynamic and post-dynamic recrystallization. CAFE proposed as an appropriate approach by combining finite element (FE) method and cellular automata (CA) for recrystallization simulation. In the current study, a random grid cellular automaton (CA), as micro-scale model, based on finite element (FE), as macro-scale method, has been used to study initial and evolving microstructural features; including nuclei densities, dislocation densities, grain size and grain boundary movement during dynamic recrystallization in a C-Mn steel. An optimized relation has been established between mechanical variables and evolving microstructure features during recrystallization and grain growth. In this model, the microstructure is defined as cells located within grains and grain boundaries while dislocations are randomly dispersed throughout microstructure. Changes of dislocation density during deformation are described considering hardening, recovery and recrystallization. Recrystallization is assumed to initiate near grain boundaries and nucleation rate was considered constant (site-saturated condition). The model produced a mathematical formulation which captured the initial and evolving microstructural entities and linked their effects to measurable macroscopic variables (e.g. stress).

Distributed power control in cellular mobile radio systems with time-varying link gains

A simple distributed power control algorithm for communication systems with mobile users and unknown time-varying link gains is proposed. We prove that the proposed algorithm is exponentially converging. Furthermore, we show that the algorithm significantly outperforms the well-known Foschini and Miljanic algorithm in the case of quickly moving mobile users.

The impact of cell formation on layout designs in cellular manufacturing

This paper provides a procedure to address all three phases of the design for cellular manufacturing namely parts/machines grouping, intra-cell and inter-cell layout designs concurrently. It provides a platform to investigate the impact of the cell formation method on intracell and inter-cell layout designs and vice versa by generating multiple efficient layout designs for different cell partitioning strategies. This approach enables the decision maker to have wider choices with regard to the different number of cells and to assess various criteria such as travelling cost, duplication of machines, space requirement against each alternative. The performance of the model is demonstrated by applying it to an example selected from literature.

The effect of artificial defects on the compressive properties of hollow sphere cellular aluminium (HSCA)

Hollow sphere cellular aluminium (HSCA) samples were fabricated by bonding together two kinds of single aluminium hollow spheres with the same outside diameter of 4 mm but different wall thicknesses of 0.1 mm and 0.3 mm, in which the hollow spheres with the thinner sphere wall thickness were used as artificial defects. Four types of HSCA samples with the same relative density but various distributions of artificial defects were prepared by simple cubic packing. For comparing, HSCA sample without defective hollow spheres inside was also prepared. The effects of the distribution of the artificial defects on the deformation behaviours and mechanical properties were investigated by compressive tests. Results indicated that the nominal stress - nominal strain curve and the deformation behavior of the HSCA samples varied with the distribution of the artificial defects in spite of the same relative density. It is therefore suggested that the deformation behavior and mechanical property of cellular materials were also significantly affected by the distribution of defects. In particular, the plateau stress of the HSCA samples increased with the decrease in number of contact points between the normal hollow spheres and the defective hollow spheres in the loading direction during deformation.

Distributed power control in cellular mobile radio systems with time-varying link gains

A simple distributed power control algorithm for communication systems with mobile users and unknown timevarying link gains is proposed. We prove that the proposed algorithm is exponentially converging. Furthermore, we show that the algorithm significantly outperforms the well-known
Foschini and Miljanic algorithm in the case of quickly moving mobile users.